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投稿日:2026年1月19日

Basic knowledge of antibacterial performance testing as specified in JIS Z 2801

What is JIS Z 2801?

JIS Z 2801 is a Japanese Industrial Standard that specifies the method for evaluating the antibacterial activity of bacteria on the surfaces of non-porous materials.
This standard is essential for industries where hygiene and anti-bacterial performance are paramount, such as in healthcare, food service, and manufacturing sectors.
It aims to ensure that products claiming to have antibacterial properties are, in fact, effective against the growth and survival of bacteria.

Why is Antibacterial Performance Important?

In everyday life, we come into contact with various surfaces that can harbor bacteria.
From doorknobs to mobile devices, these surfaces can act as breeding grounds for bacteria, which might lead to infections.
In sectors like healthcare, where sterile environments are crucial, having surfaces with reliable antibacterial performance is critical to patient safety.
Hence, testing and confirming the antibacterial effectiveness of materials used in these environments is vital.

How Antibacterial Performance Testing Works

Preparation of Test Samples

The first step in antibacterial performance testing as per JIS Z 2801 involves preparing samples of the material to be tested.
These samples are usually flat, smooth, and uniform in nature to ensure consistency in results.
An inoculum, which is a known quantity of bacteria, is applied to the surface of the test sample.

Assessing the Bacterial Load

After inoculating the samples with bacteria, these are covered and incubated for a specified time and temperature.
This process allows the bacteria to interact with the material’s surface, thereby assessing the effectiveness of the antibacterial properties.
Typically, the incubation period is 24 hours at a temperature of around 35°C.
Post incubation, the bacteria on the material surface are collected and quantified.
The reduction in bacterial load comparing the initial count to post-incubation is measured to determine the antibacterial performance.

Understanding Antibacterial Testing Results

The primary metric used in evaluating the effectiveness of the material in the JIS Z 2801 test is the “antibacterial activity value,” often denoted as R.
This value is calculated based on a reduction in the number of bacteria after a given time compared to the initial number.
An R value of 2 or more indicates effective antibacterial performance, which suggests at least a 99% reduction in bacterial count.
A higher R value signifies enhanced antibacterial properties.

Applications of JIS Z 2801 Testing

Manufacturing and Consumer Goods

This standard is widely applied in industries producing consumer goods like kitchenware, bathroom fixtures, and smartphones.
By ensuring their products have antibacterial surfaces, manufacturers can market these goods as safer for consumers, thus offering added value.

Healthcare Industry

Hospitals and medical equipment manufacturers make extensive use of JIS Z 2801 testing to ascertain the safety and efficacy of their products.
From surgical tools to coatings used on hospital walls, the presence of antibacterial surfaces reduces the risk of infections, thereby protecting patient health.

Advantages of Using JIS Z 2801 for Antibacterial Testing

JIS Z 2801 provides a standardized method, offering consistency and reliability in test results.
This standard is recognized internationally, allowing products tested under it to have broader acceptance in global markets.
Moreover, it provides consumers and industries with confidence regarding the real-world effectiveness of antibacterial claims made by manufacturers.

Limitations of JIS Z 2801

While JIS Z 2801 provides a reliable framework, it may not cover all bacteria strains or viruses.
The standard focuses primarily on Gram-negative and Gram-positive bacteria.
Therefore, manufacturers seeking comprehensive testing might need to consider additional standards for antiviral performance.
Additionally, JIS Z 2801 tests are conducted in controlled laboratory environments.
Real-world conditions can vary significantly, affecting performance.

Conclusion

Understanding and implementing JIS Z 2801 is crucial for industries aiming to create safer, more hygienic products.
By utilizing this standard, manufacturers can substantiate their antibacterial claims and offer consumers reliable, effective products.
Ultimately, this standard plays a significant role in enhancing public health and safety by ensuring materials have genuine antibacterial performance.

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